il21 (Miltenyi Biotec)
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il21
Il21, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il21/Human+IL-21%2C+research+grade/bio_rxiv__2025__09__30__675572-176-59-60
Average 94 stars, based on 13 article reviews
Il21, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il21/Human+IL-21%2C+research+grade/bio_rxiv__2025__09__30__675572-176-59-60
Average 94 stars, based on 13 article reviews
il21 - by Bioz Stars,
2026-09
94/100 stars
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other:Article Title: Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells Article Snippet: ● IL4 (PeproTech, cat. no. 0200-04-05) ● Isolation:Article Title: Discovery of rare antigen-specific TCRs via replicate profiling Article Snippet: Plates were incubated for 18 h at 37°C in the presence of 5% CO . Assays were developed according to the manufacturer’s instructions, and spots were counted using an ImmunoSpot S6 Universal Analyzer (Cellular Technology Ltd.). .. PBMCs were freshly isolated with density gradient, stained with CFSE (450 nM) and seeded in complete RPMI complete (RPMI 1640 (PANECO) + 10% heat-inactivated fetal bovine serum + 20 ng/mL IL21(SciStore)) or RP5 (RPMI 1640 (Biowest) + 5% heat-inactivated human serum (Capricorn) + 2 mM L-glutamine (Lonza) + NEAA (MP Biomedicals) + Pen/Strep + pyruvate (Biowest) + 50 ng/ml Staining:Article Title: Discovery of rare antigen-specific TCRs via replicate profiling Article Snippet: Plates were incubated for 18 h at 37°C in the presence of 5% CO . Assays were developed according to the manufacturer’s instructions, and spots were counted using an ImmunoSpot S6 Universal Analyzer (Cellular Technology Ltd.). .. PBMCs were freshly isolated with density gradient, stained with CFSE (450 nM) and seeded in complete RPMI complete (RPMI 1640 (PANECO) + 10% heat-inactivated fetal bovine serum + 20 ng/mL IL21(SciStore)) or RP5 (RPMI 1640 (Biowest) + 5% heat-inactivated human serum (Capricorn) + 2 mM L-glutamine (Lonza) + NEAA (MP Biomedicals) + Pen/Strep + pyruvate (Biowest) + 50 ng/ml Virus:Article Title: Engineering T cell receptor fusion proteins using nonviral CRISPR/Cas9 genome editing for cancer immunotherapy. Article Snippet: The second-generation lentiviral packaging system was used to produce the lentiviral vectors for CAR transduction.24,53 Following activation, bead-free T cells were incubated with lentiviral particles in RetroNectin® (Takara Bio, Kusatsu, Japan)-coated plates for 48 h at a multiplicity of infection of 50 in the presence of 200 U/ mL IL-2. .. Following removal of virus, T cell cultures were transferred to complete T cell expansion media comprising of IL-2, IL-7, IL-15, Cell Culture:Article Title: Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice Article Snippet: All cells were labeled with the viability dye Ghost Red (Tonbo Bioscience, USA). .. The antibodies used for cell culture were as follows: anti-CD3 (clone 145-2C11) and anti-CD28 (clone 37.51) from Invitrogen, USA; anti-IFN- γ (clone XMG12) and anti-IL4 (clone 11B11) from BioLegend, USA; anti-TGF β from Peprotech, USA; cytokines IL6 and Article Title: Bryostatin Activates CAR T-Cell Antigen-Non-Specific Killing (CTAK), and CAR-T NK-Like Killing for Pre-B ALL, While Blocking Cytolysis of a Burkitt Lymphoma Cell Line. Article Snippet: CART and un-transduced control (UTD) cells were cultured in TexMACSTM medium (Miltenyi Biotec) with recombinant IL-2 (premium grade, Miltenyi Biotec). .. B cells were cultured in B cell culture media (BCM), including RPMI-1640, 10% FCS, 55 mM 2-ME, 1% Pen Strep, 10 mM HEPES, 1 mM Sodium Pyruvate and 1% MEM NEAA, supplemented with recombinant human IL-2 (50 ng/ml, Miltenyi Biotec), IL-4 (10 ng/ml, PeproTech), Article Title: Interleukin-29 induces epithelial production of CXCR3A ligands and T-cell infiltration. Article Snippet: Psoriasis is considered as a model for chronic immunemediated disorders.. Th17-cells are pivotal players in those diseases.. Recently, we demonstrated that Th17-cells produce interleukin (IL)-29 and that IL-29 is highly present in psoriatic lesions. Recombinant:Article Title: IL12/18/21 Preactivation Enhances the Antitumor Efficacy of Expanded γδT Cells and Overcomes Resistance to Anti–PD-L1 Treatment Article Snippet: The following cytokines were added at the indicated concentrations to murine gdT cells: recombinant murine IL12 (10 ng/ mL; Peprotech), IL15 (50 ng/mL; Peprotech), IL18 (100 ng/mL; R&D Systems), and IL21 (30 ng/mL; eBioscience). .. For human gdT cells, recombinant human IL12 (Stemcell Technologies), IL15 (Miltenyi Biotec), IL18 (R&DSystems), and Article Title: Bryostatin Activates CAR T-Cell Antigen-Non-Specific Killing (CTAK), and CAR-T NK-Like Killing for Pre-B ALL, While Blocking Cytolysis of a Burkitt Lymphoma Cell Line. Article Snippet: CART and un-transduced control (UTD) cells were cultured in TexMACSTM medium (Miltenyi Biotec) with recombinant IL-2 (premium grade, Miltenyi Biotec). .. B cells were cultured in B cell culture media (BCM), including RPMI-1640, 10% FCS, 55 mM 2-ME, 1% Pen Strep, 10 mM HEPES, 1 mM Sodium Pyruvate and 1% MEM NEAA, supplemented with recombinant human IL-2 (50 ng/ml, Miltenyi Biotec), IL-4 (10 ng/ml, PeproTech), Concentration Assay:Article Title: IL12/18/21 Preactivation Enhances the Antitumor Efficacy of Expanded γδT Cells and Overcomes Resistance to Anti–PD-L1 Treatment Article Snippet: The following cytokines were added at the indicated concentrations to murine gdT cells: recombinant murine IL12 (10 ng/ mL; Peprotech), IL15 (50 ng/mL; Peprotech), IL18 (100 ng/mL; R&D Systems), and IL21 (30 ng/mL; eBioscience). .. For human gdT cells, recombinant human IL12 (Stemcell Technologies), IL15 (Miltenyi Biotec), IL18 (R&DSystems), and Control:Article Title: Interleukin-29 induces epithelial production of CXCR3A ligands and T-cell infiltration. Article Snippet: Psoriasis is considered as a model for chronic immunemediated disorders.. Th17-cells are pivotal players in those diseases.. Recently, we demonstrated that Th17-cells produce interleukin (IL)-29 and that IL-29 is highly present in psoriatic lesions. |
